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beta actin Monoclonal Antibody

Catalog:B-IO-10026

Geneid:P60709

Size:50/100ul

Price:880/1598

Source:Mouse

Application:WB,IHC-p WB IHC-p IF

Reactivity:H,M,R,Mk,Ch,Dg,Hm,Rb

Synonyms:ACTB,Actin,cytoplasmic 1,Beta-actin

Brand:Biozellen

beta actin Monoclonal Antibody

Catalog No. B-IO-10026                           Reactivity H,M,R,Mk,Ch,Dg,Hm,Rb

Storage Store at -20℃. Avoid freeze / thaw cycles.                      Host     Mouse

Applications WB,IHC-p                             Isotype   IgG

Note: Centrifuge before opening to ensure complete recovery of vial contents.

ImagesImmunogen Information

Immunogen Synthetic Peptide

Swissprot P60709

Synonyms ACTB,Actin,cytoplasmic 1,Beta-actin

Product Information

Calculated MW 42kDa

Observed MW 43kDa

Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.4

Purify Protein A purification

Clone No. Clone:2M3

Dilution WB 1:2000-1:5000, IHC 1:50-1:200

Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family.

Actins are highly conserved globular proteins that are involved in various

types of cell motility and are ubiquitously expressed in all eukaryotic

cells. At least six isoforms of actins are known in mammals and other

vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal

muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1

(ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are

two non-muscle actin proteins. Most actins consist of 376aa, while

ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle

cells) has only 374aa. Beta actin has been widely used as the internal

control in RT-PCR and Western Blotting as a 42-kDa protein. However,

the 41 kDa cleaved fragment of beta actin can be generated during

apoptosis process. This antibody can recognize all the actins.

圖片233.png

For Research Use Only 


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