Synonyms：GAPDH,GAPD,CDABP0047,OK/SWcl.12,Glyceraldehyde-3-phosphate dehydrogenase,GAPDH,Peptidyl-cysteine Snitrosylase GAPDH
GAPDH Monoclonal Antibody
Catalog No. B-IO-10030 Reactivity H,M,R,Mk,C,Dg,Hm,Pg,Rb,Sh,Insect,Yeast
Storage Store at -20℃. Avoid freeze / thaw cycles.
Applications WB,IHC-p,IF Host Mouse
Note: Centrifuge before opening to ensure complete recovery of vial contents.
Immunogen Synthetic Peptide
dehydrogenase,GAPDH,Peptidyl-cysteine Snitrosylase GAPDH
Observed MW 37kDa
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Purify Protein A purification
Clone No. Clone:9S5
Dilution WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.